Rat haptoglobin: Method of quantitation and response to antiarthritic therapy in collagen arthritis
Identifieur interne : 003379 ( Main/Exploration ); précédent : 003378; suivant : 003380Rat haptoglobin: Method of quantitation and response to antiarthritic therapy in collagen arthritis
Auteurs : Richard B. Gilbertsen [États-Unis]Source :
- Immunopharmacology [ 0162-3109 ] ; 1986.
English descriptors
- Teeft :
- Acute phase proteins, Acute phase reactant haptoglobin, Acute phase reactants, Adjuvant arthritis, Antiarthritic, Antiarthritic drugs, Antiarthritic therapy, Antihuman, Antirheumatic, Antirheumatic drug therapy, Antirheumatic drugs, Apr, Arthritic, Arthritic index, Arthritic rats, Arthritis, Bacterial endotoxin, Collagen, Collagen arthritis, Collagen arthritis model, Collagen model, Disease activity, Disease processes, Disease severity, Erythrocyte sedimentation rate, Goat antihuman haptoglobin, Greater relevance, Haptoglobin, Haptoglobin concentration, Haptoglobin concentrations, Haptoglobin immunodiffusion kits, Haptoglobin levels, Haptoglobin show, Human haptoglobin, Human reference serum, Human serum, Immunodiffusion, Immunological crossreactivity, Incomplete adjuvant, Mcconkey, Minimal effect, Natural bacteriostat, Nonarthritic rats, Nonsteroidal antiinflammatory drugs, Nsaid, Precipitin lines, Probert, Rabbit antihuman haptoglobin, Rabbit antihuman haptoglobin antiserum, Rat, Reactant, Rheum, Rheumatoid, Rheumatoid arthritis, Serum haptoglobin, Serum haptoglobin concentration, Serum sample, Serum samples, Therapeutic dosing protocol, Various agents, Various drugs, Weekly intervals.
Abstract
Abstract: Concentrations of the acute phase reactant haptoglobin were quantitated in the serum of rats using a commercially available antihuman haptoglobin radial immunodiffusion kit. That this antiserum reacted with rat haptoglobin was shown through the techniques of Ouchterlony immunodiffusion and immunoelectrophoresis. Haptoglobin levels were increased seven days after immunization of rats with type II collagen plus incomplete Freund's adjuvant (ICFA) and peaked on day 14. However, even six weeks post-immunization the concentration of haptoglobin was elevated in arthritic rats. A significant correlation was observed between the concentration of serum haptoglobin and the severity of disease (arthritic index) in rats immunized six weeks previously with type II collagen plus ICFA. The effects of antiinflammatory and antirheumatic therapu on arthritic index and serum haptoglobin concentration were determined using a therapeutic dosing protocol. Under these conditions, the known antiarthritic effects of nonsteroidal antiinflammatory drugs, steroids and immunosuppressive agents in this model were confirmed. Of these agents, only the nonsteroidal drugs reduced serum haptoglobin; hydrocortisone, cyclophosphamide and azathioprine elevated haptoglobin. Aurothioglucose, auranofin, and chloroquine, members of the class of disease-modifying antirheumatic drugs, had a general tendency to exacerbate disease, but had minimal effect on serum haptoglobin. D-Penicillamine had little effect on arthritic index and haptoglobin. These results suggest that, while haptoglobin levels do correlate with the intensity of hindpaw swelling, measurement of haptoglobin may not be an accurate indicator of the underlying disease processes in the collagen arthritis model.
Url:
DOI: 10.1016/0162-3109(86)90026-3
Affiliations:
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Rat haptoglobin: Method of quantitation and response to antiarthritic therapy in collagen arthritis</title><author><name sortKey="Gilbertsen, Richard B" sort="Gilbertsen, Richard B" uniqKey="Gilbertsen R" first="Richard B." last="Gilbertsen">Richard B. 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Gilbertsen</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology, Immunopharmacology Section, Warner-Lambert/Parke-Davis Pharmaceutical Research, Ann Arbor, MI 48105</wicri:regionArea><placeName><region type="state">Michigan</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Immunopharmacology</title><title level="j" type="abbrev">IMPHAR</title><idno type="ISSN">0162-3109</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1986">1986</date><biblScope unit="volume">11</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="69">69</biblScope><biblScope unit="page" to="77">77</biblScope></imprint><idno type="ISSN">0162-3109</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0162-3109</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acute phase proteins</term><term>Acute phase reactant haptoglobin</term><term>Acute phase reactants</term><term>Adjuvant arthritis</term><term>Antiarthritic</term><term>Antiarthritic drugs</term><term>Antiarthritic therapy</term><term>Antihuman</term><term>Antirheumatic</term><term>Antirheumatic drug therapy</term><term>Antirheumatic drugs</term><term>Apr</term><term>Arthritic</term><term>Arthritic index</term><term>Arthritic rats</term><term>Arthritis</term><term>Bacterial endotoxin</term><term>Collagen</term><term>Collagen arthritis</term><term>Collagen arthritis model</term><term>Collagen model</term><term>Disease activity</term><term>Disease processes</term><term>Disease severity</term><term>Erythrocyte sedimentation rate</term><term>Goat antihuman haptoglobin</term><term>Greater relevance</term><term>Haptoglobin</term><term>Haptoglobin concentration</term><term>Haptoglobin concentrations</term><term>Haptoglobin immunodiffusion kits</term><term>Haptoglobin levels</term><term>Haptoglobin show</term><term>Human haptoglobin</term><term>Human reference serum</term><term>Human serum</term><term>Immunodiffusion</term><term>Immunological crossreactivity</term><term>Incomplete adjuvant</term><term>Mcconkey</term><term>Minimal effect</term><term>Natural bacteriostat</term><term>Nonarthritic rats</term><term>Nonsteroidal antiinflammatory drugs</term><term>Nsaid</term><term>Precipitin lines</term><term>Probert</term><term>Rabbit antihuman haptoglobin</term><term>Rabbit antihuman haptoglobin antiserum</term><term>Rat</term><term>Reactant</term><term>Rheum</term><term>Rheumatoid</term><term>Rheumatoid arthritis</term><term>Serum haptoglobin</term><term>Serum haptoglobin concentration</term><term>Serum sample</term><term>Serum samples</term><term>Therapeutic dosing protocol</term><term>Various agents</term><term>Various drugs</term><term>Weekly intervals</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Concentrations of the acute phase reactant haptoglobin were quantitated in the serum of rats using a commercially available antihuman haptoglobin radial immunodiffusion kit. That this antiserum reacted with rat haptoglobin was shown through the techniques of Ouchterlony immunodiffusion and immunoelectrophoresis. Haptoglobin levels were increased seven days after immunization of rats with type II collagen plus incomplete Freund's adjuvant (ICFA) and peaked on day 14. However, even six weeks post-immunization the concentration of haptoglobin was elevated in arthritic rats. A significant correlation was observed between the concentration of serum haptoglobin and the severity of disease (arthritic index) in rats immunized six weeks previously with type II collagen plus ICFA. The effects of antiinflammatory and antirheumatic therapu on arthritic index and serum haptoglobin concentration were determined using a therapeutic dosing protocol. Under these conditions, the known antiarthritic effects of nonsteroidal antiinflammatory drugs, steroids and immunosuppressive agents in this model were confirmed. Of these agents, only the nonsteroidal drugs reduced serum haptoglobin; hydrocortisone, cyclophosphamide and azathioprine elevated haptoglobin. Aurothioglucose, auranofin, and chloroquine, members of the class of disease-modifying antirheumatic drugs, had a general tendency to exacerbate disease, but had minimal effect on serum haptoglobin. D-Penicillamine had little effect on arthritic index and haptoglobin. These results suggest that, while haptoglobin levels do correlate with the intensity of hindpaw swelling, measurement of haptoglobin may not be an accurate indicator of the underlying disease processes in the collagen arthritis model.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Michigan</li></region></list><tree><country name="États-Unis"><region name="Michigan"><name sortKey="Gilbertsen, Richard B" sort="Gilbertsen, Richard B" uniqKey="Gilbertsen R" first="Richard B." last="Gilbertsen">Richard B. Gilbertsen</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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